05, 20 · Tumor Formation in p53 Mutant Zebrafish. e importance of p53 in cancer is highlighted by e fact at it is e most frequently mutated tumor suppressor gene in human cancers, wi more an half of solid tumors harboring mutations in e p53 locus. In many o er tumors, p53 is inactivated by more indirect mechanisms, including MDM2 amplification and p14ARF deletion Cited by: 70. Tumor Formation in p53 Mutant Zebrafish. e importance of p53 in cancer is highlighted by e fact at it is e most frequently mutated tumor suppressor gene in human cancers, wi more an half of solid tumors harboring mutations in e p53 locus. In many o er tumors, p53 is inactivated by more indirect mechanisms, including MDM2 amplification and p14ARF deletion (Vogelstein et al. 2000).Cited by: 70. 11, 2005 · TP53 is e most frequently mutated tumor suppressor gene in human cancer, wi nearly 50 of all tumors exhibiting a loss-of-function mutation. To fur er elucidate e genetic pa ways involving TP53 and cancer, we have exploited e zebrafish, a powerful vertebrate model system at is amenable to whole-genome ford-genetic analysis and syn etic-le al screens. Zebrafish lines Cited by: 559. 01, 20 · p53 protein is an important regulation factor at can bind to p53 mRNA to regulate its translation in human and murine. To determine if a similar interaction exists in zebrafish and if e interaction affects zebrafish development, we cloned and expressed p53 protein from zebrafish in Escherichia coli.Soluble p53 protein wi high purity was successfully obtained using e optimized Cited by: 5. Apr 24, · (A) UV cross-linking was performed to detect e binding of zebrafish p53 protein wi its mRNA in vitro as described in e Materials and Me ods. p53 protein (1 μg) was incubated wi Biotin-labeled full- leng p53 mRNA (lane1), p53 3′UTR mRNA(lane 2), p53 5′UTR+ORF mRNA(lane 3). (B) Expression of ectopic his-p53 in zebrafish embryos. 5291 University of Oregon Eugene, OR 97403-5291 [email protected] @ZFINmod. p53 Antibody Cocktail. Advanced Verification. 8 References Advanced Verification. 8 References figures. Target. Human Mouse Rhesus monkey. Details. Mouse Cocktail. Application. ChIP IHC (P) IP WB. Price. Online offer: Cat MA5-14067. 500 µL. Compare. Invitrogen. p53 Monoclonal Antibody . Zebrafish (Danio rerio), a minnow-like freshwater fish, has emerged as a popular animal model for research and development.Due to eir striking genomic similarity to humans, regenerative abilities, and shortened life cycle, zebrafish have become an ideal tool for studying developmental processes, gene expression, and underlying mechanisms of cancer and o er diseases. About ese Antibodies All antibodies listed on is page were generated by researchers at e Institute of Neuroscience, University of Oregon (Liu et al., 2003, Amacher, et al., 2002, and Trevarrow et al., 1990). e antibodies were generated wi BALB/c mice against adult zebrafish protein, and clones were selected based on e specific staining patterns obtained wi particular clones. p53 (121). p53R2 (11). Mutant p53 (9). p53 DINP1/TP53INP1 (5). p53 AIP1 (3). Apoptosis Antibody Array (1). Cyclin E2+PAK1 + PAK2 + PAK3+CaMKII+PHKG2+MAP4K5+MAP4K3/GLK+CCNB3 (1). CYP1A1+CYP1B1 (1). Human MAPK Phosphorylation Antibody Array (1). IL-6+Glutamine Syn etase+ADAM17+Glucocorticoid Receptor+Mineralocorticoid Receptor+Androgen Recepto (1). . Zebrafish cancers can be induced by chemical mutagens (such as 7,12-dime ylbenz(a)an racene [DMBA]), specific genetic mutations, or oncogene transgenesis. 21 For example, expression of e BRAF V600E mutation, e most common mutation in human nevi and melanoma, is sufficient to induce ectopic nevi in zebrafish, and can collaborate wi p53. Our results demonstrate at zebrafish p53 protein can readily be induced in embryos and detected using ese new antibody tools, which will increase e usefulness of zebrafish as a model. Custom antibody development and commercial partnerships to advance your diagnostic and erapeutic discovery. Create custom solutions wi us Hamster, Dog, Human, Pig, Xenopus laevis, Monkey, Zebrafish. Conjugate: Unconjugated. Anti-Caspase-3 antibody (ab13847) Reviews (53) Specific References (596) Description: Rabbit polyclonal to Caspase. 208 reviews. Compare Anti-Zebrafish/Fish Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Apr 13, · p53 Antibody (A-1) is a mouse monoclonal IgG 1 (kappa light chain) provided at 200 µg/ml. specific for an epitope mapping between amino acids 353-391 at e C-terminus of p53 of rat origin. Anti-p53 Antibody (A-1) is recommended for detection of p53 of mouse, rat and human origin by WB, IP, IF and ELISA4.4/5(27). In is system e p53 gene is identified as a p53-responsive gene itself. Our results demonstrate at zebrafish p53 protein can readily be induced in embryos and detected using ese new antibody tools, which will increase e usefulness of zebrafish as a model in compound-based screening for el drugs in cancer research. Compare Anti-CD209a Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 17, · Typically, e induction of DNA damage in zebrafish embryos by γ-irradiation results in p53 stabilization. 37 is is observed by Western blotting wi a zebrafish p53-specific antibody in 4 dpf wild-type embryos treated wi 25 Gy of γ-irradiation after 6 hours (Figure 3A). In y , e 17 International p53 Workshop was held in Singapore. Wi an attendance of over 300 scientists from all over e world, e meeting was an outstanding success and many new observations about e p53 pa way were discussed for e first time. 12, 2005 · 209 Accesses. 51 polδ1-p53 double-deficient zebrafish are blocked for min in immunostaining blocking solution, 32 followed by subsequent incubation wi e pri y anti-BrdU antibody. Products added to your personal shortlist can be viewed by clicking e 'View your shortlist' link at e top of e results table. To add a product, click e + link. to remove. Understanding p53 functions rough p53 antibodies O er tools. Mutalyzer, a package to support checks of sequence variant nomenclature according to e guidelines of e Human Genome Variation Society. Resources for e systematic analysis of p53 interactions wi DNA and o er transcription factors. Search results for p53 at Sigma-Aldrich. Species Human (7158), Species Rat (296099), Species Zebrafish (324972), Species Horse (0056322), Species domestic cat ( 98873), Species dog (478274), Species chicken (415589), Species naked mole-rat (172 77), Species Domestic Rabbit (0328680), Species domestic guinea pig (0717584), Species cow (509111), Species sheep . P53 antibody Rabbit Polyclonal from Proteintech validated in Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), CoImmunoprecipitation (IP), chImmunoprecipitation (IP), Enzyme-linked Immunosorbent Assay (ELISA) applications. is antibody reacts wi human, rat, mouse samples. Cat.No. 442-1-AP. KD/KO Validated. Phospho-p53 (Ser15) Antibody detects endogenous levels of p53 only when phosphorylated at serine 15. e antibody does not cross-react wi p53 phosphorylated at o er sites. Species Reactivity: Human, Mouse, Rat, Monkey. Species predicted to react . Ribosomal biogenesis involves e processing of pre-ribosomal RNA. A deficiency of some ribosomal proteins (RPs) impairs processing and causes Diamond Blackfan anemia (DBA), which is associated wi anemia, congenital malformations and cancer. p53 mediates many features of DBA, but e mechanism of p53 activation remains unclear. Ano er hall k of DBA is e upregulation of adenosine. Extracts ( μg) from horionated and deyolked embryos, obtained as indicated above for HEK293 cells, were probed wi a 50 dilution of e monoclonal antibody LLzp53-9E, which is specific to zebrafish P53 (Maclnnes et al., 2008). Membranes were en reprobed wi a 1:5000 dilution of a commercial rabbit antibody to histone 3 (ab1791. Alternative Name p53 (TP53) (TP53 Antibody Abstract) Background P53, also known as protein 53 (TP53), is a transcription factor at regulates e cell cycle and hence functions as a tumor suppressor in a variety of tissue types and is involved in regulating cell grow, replication and apoptosis. Morpholinos against p53 did not rescue e phenotype, nor were zebrafish wi a mutant p53-null allele resistant to PCB126 toxicity. Morpholino knockdown of cardiac troponin T, which blocks e onset of cardiac function, prevented e PCB126-induced cardiac . 29, · Chen, J. et al. p53 Isoform delta113p53 is a p53 target gene at antagonizes p53 apoptotic activity via BclxL activation in zebrafish. Genes Dev. 23, 278–290 (2009). Fremont, CA, ober 04, 2009 AnaSpec has announced e release of a zebrafish specific p53 antibody, e Z-Fish Anti-p53. is antibody was tested in IHC using a 48-hpf zebrafish embryo tail. Choose from a large selection of p53 antibodies at Bio-Rad. Simply filter by specificity, host, clone or application to find e exact antibody to suit your needs. 425805 a0dcbffd-572a-4aa0-8ffe-00a93a388c8c. 02, 2006 · To investigate downstream p53-dependent transcriptional responses in e zebrafish, we examined e expression of p53 target genes such as p21 WAF 1/CIP 1 and cyclin D1, based on eir central role in cell-cycle arrest. bax, for its involvement in e apoptosis pa way. and p53 and mdm2 to explore e feedback loops downstream of p53 (Figure 4C. Invitrogen Anti-Phospho-p53 (Ser15) Recombinant Monoclonal (14H61L24), Catalog 700439. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (Flow), ChIP assay (ChIP) and Fluorescence Resonance Energy Transfer (FRET) (FRET) applications. is antibody reacts wi Human samples. Supplied as 0 µg purified antibody (0.5 mg/mL). Rabbit Polyclonal p53 antibody Tyr216 for ELISA, IHC (p), WB. Order anti-p53 antibody ABIN3187854. p53, also known as Tp53, is a DNA-binding protein which belongs to e p53 family. It contains transcription activation, DNA-binding, and oligomerization domains. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 (TP53) is a transcription factor whose protein. Western blot was performed as described previously. 21 e following pri y antibodies were used in is study: anti-BCAS2 antibody (GeneTex, Cat GTX115390), anti-GAPDH antibody (Dia-an, Cat 2058), anti-p53 antibody (GeneTex, Cat GTX128135), and anti-γH2AX antibody (Cell Signaling Technology, Cat 2577). e images were captured by using. Recombinant Mouse Anti-Zebrafish tp53 Antibody (ZFp53-9.1) (CAT: MD-ZBF-YC009) is product is a mouse monoclonal antibody at specifically targeting tp53 from zebrafish, which is a model organism for e study of human disease. It was reported at 28 of zebrafish p53 homozygous mutant fish developed tumors by 16.5 mon s, and at more an 50 of ese tumors occurred in e eyes (Berghmans et al., 2005). Because it is possible at such retinal tumors are derived from retinal cells in which DNA damage fails to be repaired, e ATM-Chk2-p53 pa way might suppress. Here, we show at, in zebrafish, a genetic mutation of rpc9 leads to e impairment of HSPC survival in e CHT, and ese hematopoietic defects can be rescued by downregulation of p53 (tp53 – Zebrafish Information Network). erefore, ese findings fur er our understanding on e role of Rpc9 as a component of RNA polymerase III. p53 restrains transposons in Drosophila. Previously, we showed at lesions in e Drosophila piwi-interacting RNA (piRNA) pa way consistently triggered p53 activity (Wylie et al. ), raising e possibility at p53 might function to restrain retrotransposons at are targets for piRNA suppression.To address is possibility, we examined e expression of TAHRE elements in p53 − flies. p53 antibody used for immunocytostaining application wi DAB detection on HeLa cells treated or not wi Anredera cordifolia leaf extract or 40um cisplatin - 5 minutes fixation, 15 minutes incubation wi H2O2, 1 hour pri y incubation, detection wi biotinylated goat anti-polyvalent secondary antibody and DAB staining followed by counter. Interestingly, p53‐dependent apoptosis has recently also been reported in a zebrafish model of Alzheimer's disease (Campbell et al. 2006). Future work should concentrate on e identification of e upstream triggers of p53 activation in DJ‐1 deficiency, but also fur er elucidate e exact downstream mechanisms of combined DJ‐1. Mutant p53 is a cancer-specific target for pharmacologic intervention. We show at histone deacetylase inhibitors such as FR901228 and trichostatin A completely depleted mutant p53 in cancer cell lines. is depletion was preceded by induction of p53-regulated transcription. In cells wi mutant p53 pretreated wi histone deacetylase inhibitors, DNA damage fur er enhanced e p53 trans. Activation of apoptosis is believed to be critical for e role of p53 as a tumor suppressor. Here, we report a new mouse strain carrying a human p53 transgene in e mouse p53-null background. Expression of human p53 in ese mice was comparable wi wild-type murine p53. however, transactivation, induction of apoptosis, and G1-S checkpoint, but not transrepression or regulation of. FITC anti-p53 Antibody - p53 is a 53 kD protein at forms tetramers and functions as a tumor suppressor and transcriptional activator of genes at inhibit grow and/or invasion, cell cycle checkpoint after irradiation, DNA repair, apoptotic induction, signal transduction, and cell adhesion.